产品货号：
Z21616
中文名称：
N4BP1抗体
英文名称：
Anti-N4BP1 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-N4BP1 Antibody |
| 指标别名 | N4BP1;KIAA0615;NEDD4-binding protein 1;N4BP1 |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | WB,ICC/IF,FCM(Intracellular),Direct ELISA |
| 基因名称 | N4BP1 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human N4BP1 recombinant protein (Position:M1-K885). |
| 实际分子量 | 110KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin–protein ligase (E3),Nedd4.Here,we describe a previously unrecognized functional interaction between N4BP1 and Itch,a Nedd4 structurally related E3,which contains four WW domains,conferring substrate-binding activity.We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates.In particular,we found that N4BP1 and p73α,a target of Itch-mediated ubiquitin/proteasome proteolysis,share the same binding site.By competing with p73α for binding to the WW2 domain,N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73α and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays. |
| Uniprot ID | O75113 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for ICC. |
| 基因名全称 | NEDD4 binding protein 1 |
| 蛋白名全称 | NEDD4-binding protein 1 |
| 推荐稀释比 | Western blot (WB) 1:500～2000 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow Cytometry(Intracellular) 1～3μg/1×106 cells Direct ELISA 1:100～1000 |
相关搜索：N4BP1抗体，N4BP1多抗，Anti-N4BP1 Antibody